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KMID : 1134820150440030324
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Journal of the Korean Society of Food Science and Nutrition
2015 Volume.44 No. 3 p.324 ~ p.330
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Inhibition of Type II Diabetes in ob/ob Mice and Enhancement of Mitochodrial Biogenesis in C2C12 Myotubes by Korean Mistletoe Extract
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Jung Hoe-Yune
Yoo Yung-Choon
Kim In-Bo
Sung Nak-Yun
Choi Ok-Byung
Choi Bo-Hwa
Kim Jong-Bae
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Abstract
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In this study, the anti-diabetic activity of a cold water extract of Korean mistletoe (KME) was investigated in C57BL/6J Lep ob (ob/ob) mice. Oral administration of KME (50 or 100 mg/kg/d) significantly inhibited the level of blood glucose of ob/ob mice after 5 days from the beginning of KME treatment. And the anti-diabetic effect of KME was stabilized 10 days after oral administration, showing a substantial reduction of blood glucose levels by more than 20% as compared with control mice. The results of oral glucose tolerance test (OGTT) revealed that oral administration of KME gave rise to a remarkable improvement in overall glucose response. Oral administration of KME in ob/ob diabetic mice also significantly reduced blood total cholesterol (TCHO) and triglyceride (TG) levels compared with the diabetic control mice. Moreover, in an in vitro experiment using C2C12 myotubes, treatment of KME prominently increased glucose uptake. Interestingly, KME significantly increased the expression of peroxisome proliferator-activated receptor gamma coactivator 1-α (PGC-1α), a head regulator of mitochondrial biogenesis and oxidative metabolism, and PGC-1α-associated genes such as glucose transporter type 4 (GLUT4), estrogen-related receptor-α (ERR-α), nuclear respiratory factor-1 (NRF-1), and mitochondrial transcription factor A (TmfA) in C2C12 cells. These results suggest that KME has potential as a novel therapeutic agent for diabetes, and its anti-diabetic activity may be related to the regulation of mitochondrial biogenesis.
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KEYWORD
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Korean mistletoe, diabetes, metabolic syndrome, mitochondria, ob/ob mice
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